TE 357 UNIT 4 TE 4.1 A UNDERSTANDING GROWTH AND DEVELOPMENT CHAPTER 4.1 DNA: THE MOLECULE OF LIFE ENGAGE , page 250 Group Lab Activity: Extracting DNA Advance Preparation: Chill 90% isopropyl alcohol in freezer or ice chest. Make sure alcohol remains on ice throughout the investigation. Label 100 mL beakers for each lab group: “ salt solution, ” “ detergent solution, ” and “ alcohol. ” Prepare a 9% salt solution prior to class. Place 9 grams of NaCl into 100 mL of distilled water. Distribute approximately 10 mL to each lab group. Prepare a 25% detergent solution. Pour 25 mL liquid dish detergent into a 250 mL beaker. Add 75 mL of distilled water. Distribute approximately 10 mL to each lab group. Prepare a split green pea puree prior to class. Add to blender: 100 mL split green peas and 200 mL cold water. Blend for 15 seconds. Pour through strainer into larger beaker. Distribute approximately 20 mL to each lab group. Split Green Pea Puree Materials: – Blender – Cold water – Bag of split green peas – Large beaker – Strainer Materials (per student): – Safety goggles – Test tube stopper – Small paper cup – Glass stir rod – 2 Test tubes – Microcentrifuge tube (optional) Materials (per group): – 4 100 mL Beakers – Measuring spoons (mL) – 3 Plastic transfer pipettes – 10 mL Graduated cylinder – 2 Test tube racks – Split green pea puree – Meat tenderizer – 10 mL 9% Salt solution – Bottled water – 10 mL 25% Detergent solution – BLMs 115–117, Extracting DNA – 50 mL 90% Isopropyl alcohol, chilled
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